Influence of clearing agent on immunohistochemical staining of paraffin-embedded tissue.
نویسنده
چکیده
منابع مشابه
Comparison of Polymerase Chain Reaction, Ziehl-Neelsen Staining and Histopathologic Findings in Formalin-fixed, Paraffin-Embedded Tissue Specimens for Diagnosis of Tuberculosis
Background and Objective: Tuberculosis is still a major health problem, involving about 1/3 of the world´s population. Diagnosis is difficult when we only use Ziehl-Neelson staining. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study was to compare PCR, Zieh-Neelsen staining and histopathologic findings in diagnosis of ...
متن کاملComparison Between Xylene And Coconut Oil In Tissue Processing
Background: Clearing is an important step in the preparation of histological sections, that removes alcohol and other dehydrants from tissues prior to infiltration of the embedding material (usually paraffin wax).Different types of clearing agents are chloroform, Xylene, Toluene, Paraffin, Methyl benzoate and methyl salicylate & Citrus fruit oils.The commonly used clearing agent is xylene that ...
متن کاملThe influence of tissue pretreatment on the immunohistochemical demonstration of type I and III collagens and tenascin in fetal human tooth germs.
The influence of tissue pretreatment on the PAP immunostaining for type I and III collagens and tenascin was studied in formalin-fixed and paraffin-embedded human tooth germs at the 24th and 25th weeks of fetal life. Three variables were considered: the type of buffer used (PBS or Tris), pepsin digestion and the use of normal serum as a blocking agent prior to immunostaining. All three proteins...
متن کاملImmunohistochemical Detection of the Human Herpes Virus 8 (HHV8) Latent Nuclear Antigen-1 in Kapasi Sarcoma Cases
Background and Objectives: The molecular genetic of the human herpes virus 8 (HHV8) has now been characterized the pathogenesis of kaposi sarcoma (KS). This study attempted to determine the rate of HHU-8 infection in KS in an Iraniann cross sectional study. Method & material: In this cross-sectional study, we used paraffin-embedded specimens of 54 clinically well-characterized kaposi sarcom...
متن کاملThe Comparison Between Microwave and Autoclave as Antigen Retrieval Methods for Immunohistochemical Detection of CD15 and CD30 in Hodgkin’s Lymphoma
Background and Objective: Hodgkin's lymphoma is a potentially curable hematologic malignancy with difficulty in its diagnosis especially in atypical cases even in expert hands. Today, immunohistochemistry plays a significant role in the diagnosis of it especially applying the anti-CD15 and anti-CD30 antibodies. The negativity of CD15 can be reduced by antigen retrieval for meth...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical pathology
دوره 34 1 شماره
صفحات -
تاریخ انتشار 1981